Figure 7

Expression levels of candidate IRs in male and female S. litura antennae measured in single-end RNA-seqence (A) and RT-qPCR (B). In single-end RNA-seqence, vertical axis means the log scale of RPKM value to 10. The significant difference between female and male was justified by method of Audic and Claverie (1997) and indicated by symbol “*” (FDR < 0.01 and P < 0.05). In RT-qPCR, vertical axis means log scale of female gene expression to 10. Female gene expression was calculated by the 2^-∆∆Cq algorithm with male as control and GAPDH and UCCR as reference genes. The significant difference was ascertained by Students t test. Symbol “*” means the significant difference between female and male (P < 0.05). Error bars signify SD”.