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Figure 1 | BMC Genomics

Figure 1

From: One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)

Figure 1

Schematic of PITT-mediated targeted insertion using the new seed mice, TOKOM-3. Cre-PITT (via mutant loxP sites) and PhiC31-PITT (via attB/P site) confer insertion of the donor vector containing. DNA Of Interest (DOI), into a target site in the genome, to generate TIex allele 1 or 2 respectively. DOI shown in this example is tdTomato-poly(A) cassette in this figure. Introduction of FLP removes extra sequence in the TIex allele 1 and 4, resulting in generation of TIΔex allele. See Additional file 1: Figures S1, S3 and S4 for other possible outcomes recombination. GTNOP: a cassette containing “eGFP-T2A-Neomycin resistant gene-hOCT4-PolyA”.

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