Mutations and genomic islands can explain the strain dependency of sugar utilization in 21 strains of Propionibacterium freudenreichii

Table 3 Sugar degradation abilities listed for 21 sequenced strains of Propionibacterium freudenreichii

			binary manner			quantitative manner
CIRM-BIA strain names	Actalia strain names	nitrate	lactose	xylitol	melibiose	gluconate	ribose	D-fructose	arbutine	L-arabitol	L-arabinose	number of degraded sugars	number of differentially degraded sugars
1		0	1	0	0	0.75	0	0.75	0	0.75	0.75	13	5
9		0	1	0	0	0.75	0.75	1	0	0.5	0.75	14	6
118		0	1	0	0	0	0.75	0.75	0	0	0	13	5
119		1	1	0	0	0.75	0	1	0.75	0	1	13	5
121		1	0	0	0	0.75	0.25	1	0	0.75	0.50	13	5
122		1	1	0	0	0	1	0.50	0	0.25	1	14	6
123		0	1	0	0	0.75	0.50	0.75	1	0	0.75	14	6
125	ITG P14	0	1	0	0	0	0.25	0	0.75	0	0.75	12	4
127	ITG P18	1	0	0	0	0.50	0.50	0.75	0	0.50	0.50	13	5
129	ITG P20	0	1	0	0	0.75	0	1	0.75	0	1	13	5
134		0	1	0	0	0.75	0	0.75	0	0.75	1	13	5
135		0	1	0	0	0.75	0	0.75	0.75	0	0.25	12	4
138	ITG P9	1	1	1	0	0.75	0	0.75	0	0	1	13	5
139	ITG P23	1	1	0	0	0.50	0	0.75	0	0.50	0.75	13	5
456		0	1	0	0	0.75	0	0.75	0	0	0	11	3
508		0	1	0	0	0	0.75	0.75	0	0	0	11	3
512		0	1	0	0	0.50	0.75	0.75	0	0.75	0.75	14	6
513		0	1	1	1	0.50	0	1	0	0.50	1	15	7
514		1	0	0	0	0.50	0	0	0	0.75	1	11	3
516		1	1	0	0	0.75	0.75	0.75	0	0.75	1	14	6
527		1	0	0	0	0.75	0	0	0	0	0.75	10	2
1025	ITG P1	0	1	0	0	0.75	0.25	0.75	1	0	0.75	13	5

Their ability to reduce nitrate was assessed as described in Materials and Methods. Their sugar degradation capability was assessed using Gallery API 50 CH. 1 means that the strain efficiently fermented the sugar, 0 means that the strain was unable to degrade the sugar. Intermediate phenotypes were scored 0.25, 0.50, 0.75, indicated that the sugar was partially used by the strain.
Footnote: Glucose, glycerol, mannose, galactose, inositol, adonitol, erythritol, esculine were degraded by all studied strains.
Arbutine is not a sugar but a glycosylated quinone. No further efforts were made to reconstruct its degradation pathway.

ISSN: 1471-2164