Figure 4

Experimental analyses of polycistronic unit 10 (TU_10) by RT-PCR. A: Structure and characteristics of class 2 terminator w_014, predicted by the software WebGesTer. B: agarose gel electrophoresis of RT-PCR products obtained from primers used to analyse expression in TU_10. The reactions were performed in the presence (+) and absence (−) of reverse transcriptase. Lanes 2, 3, 4 and 5 represent the reaction with the primer positioned upstream of the terminator. Lanes 6, 7, 8 and 9 represent the reaction with the primer positioned downstream of the terminator. Results of the reaction with positive cDNA are presented in lanes 3 and 7. Control reactions (DNA) using genomic DNA as the template are presented in lanes 2 and 6. Lanes 4 and 8 represent the control reaction of cDNA synthesis while lanes 5 and 9 are the control of the PCR reaction. Lane 1 contains a 100 bp ladder.