Figure 3

Experimental validation of predicted and conserved wheat miRNAs. a) Pre-miRNA secondary structure of miRNA candidates experimentally validated by northern in the investigated libraries; b) northern blot of predicted miRNAs in common between MiRdup* and MIRcheck (CM*M) as well as specifically predicted with MiRdup* tool (SM*); c) northern blot of miRNA candidates identified by both sequence homology against miRBase (conserved miRNAs) and predicted in common between MIRcheck and MiRdup*. Ethidium bromide staining of the rRNAs is shown as gel loading control. L0 represents the control library for Al treatment (L8) in spring wheat Bounty which was not sequenced. The numbers between the parentheses correspond to the expression rank among the 199 predicted miRNAs. The lower value corresponds to the higher read abundance. For more information about the libraries and conditions see Additional file 1: Method S1 and Additional file 2: Table S1.