Table 2 Characteristics of selected miRNAs using MiRdup* and MIRcheck validated by northern blot
ID | MiRNAs | A | B | C | D | Associated conditions | Relevant targets (with uniref ID) | Training dataset |
|---|---|---|---|---|---|---|---|---|
Predicted MiRNAs in common with MiRdup* and MIRcheck | ||||||||
apMir_20602 | GUCAUCUAUAUUGGAACGGAG | 1 | 1 | 1 | 5 | Salt, floral transition and flowering | Glutathione peroxidase (Q9SME6), putative phosphatase phospho1 (M8CZ66) | BM |
apMir_19980 | AUAGCAUCAUCCAUCCUACCA | 3 | 1 | 3 | 6 | Al, floral transition and flowering | Putative membrane-associated protein (gi|22548307|gb|BU100508.1|BU100508) | BPM |
apMir_14769 | GUUGUCAUAUAUGUAUUGA | 2 | 1 | 2 | 6 | Cold, Al and salt | Putative RSH disease resistance-related protein (Q8H5X7), T-complex protein 1 subunit alpha (I3RZC6) | M |
apMir_21052 | UGAGAUGAGAUUACCCAAUAC | 3 | 2 | 4 | 7 | Cold, floral transition and flowering | NA | P |
Predicted MiRNAs specific to MiRdup* | ||||||||
apMir_16808 | CAUCGAUCAUCCAUCACCC | 2 | 5 | 6 | 7 | Not differentially expressed | Dehydrin, (CD909074, TA50415_4565), Phosphorylase (Q84P16) | B |
apMir_86202 | AGGGUCGGCCAGCGGUGCGGCCCGU | 4 | 2, 4 | 8 | 6 | Cold, floral transition and Al | NA | BM |
apMir_54471 | UCAGUCAUAAUCCGGCAC | 3 | 1 | 3 | 7 | Salt, Al and floral transition | NA | MP |
Conserved miRNAs predicted in common by MiRdup* and MIRcheck | ||||||||
apMir_22246 (tae-miR160) | UGCCUGGCUCCCUGUAUGCCA | 3 | 1 | 3 | 9 | Cold, salt, Al, floral transition and flowering | Auxin response factor (M8BC98), Auxin responsive protein (R7WEP7) | BPM |
apMir_20968 (miR395-21) | UGAAGUGUUUGGGGGAACUCU | 2 | 1 | 2 | 8 | Cold, salt, Al, flowering | Bifunctional 3′-phosphoadenosine 5′-phosphosulfate synthase (M7ZFX2), ATP sulfurylase (M9T1P9) | BMP |