Table 1 Summary of sequence data from whole genome sequencing of four Cryptosporidium hominis specimens in comparison with data from the published C. hominis (TU502) and C. parvum (IOWA) genomes
Specimen (gp60 subtype) | Technique | Total nucleotides | Total sequence reads | Assembly | Average coverage (fold) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
# of Contigs | Length (bp) | Mean (bp) | Minimum (bp) | Maximum (bp) | N50 (bp) | |||||
30976 (IaA28R4) | Illumina Genome Analyzer IIx 100Â bp paired end | 5,780,028,818 | 64,449,544 | 6,140 | 22,133,082 | 3,605 | 502 | 1,279,890 | 145,968 | 257 |
37999 (IbA10G2) | Illumina Genome Analyzer IIx 100Â bp paired end | 2,798,259,889 | 30,886,077 | 78 | 9,054,010 | 116,077 | 510 | 1,029,232 | 406,678 | 307 |
33537 (IaA28R4) | 454 GS-FLX Titanium | 431,742,212 | 1,157,140 | 1,464 | 14,065,231 | 9,607 | 501 | 154,507 | 27,749 | 31 |
30974 (IbA10G2) | 454 GS-FLX Titanium | 382,520,957 | 1,048,412 | 443 | 8,841,752 | 19,959 | 513 | 325,032 | 78,110 | 43 |
C. hominis TU502 (IaA25R3) | Sanger | - | - | 1,422 | 8,743,570 | 6,149 | 251 | 90,444 | 14,504 | 12 |
C. parvum IOWA (IIaA15G2R1) | Sanger | - | - | 18 | 9,102,324 | 504,874 | 17,388 | 1,278,458 | 1,014,526 | 13 |