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Figure 3 | BMC Genomics

Figure 3

From: The pathogenic human Torsin A in Drosophila activates the unfolded protein response and increases susceptibility to oxidative stress

Figure 3

Transcripts of HSC3, HSP22, Tsf1 and IRE1-dependent spliced Xbp1 and Xbp1-eGFP signals were significantly increased in the HTorAΔE-expressing brains. Quantitative-RT-PCR results of genes encoding proteins displaying the largest alterations in expression in the 2-DE analysis. (A) Only the transcripts of heat shock protein cognate 3 (hsc3) but not those of hsc4 and hsc5 were significantly increased in the HTorAΔE flies. (B) The transcripts of transferrin1 (tsf1), heat shock protein 22 (hsp22), and sarcoplasimc calcium binding protein (scp1) were not changed. (C) The amount of the IRE1-dependent spliced form of Xbp1 mRNA (Xbp1 -23 bp) was increased in the HTorAΔE brains. (D) Compared with HTorAWT, expression of HTorAΔE induced increased expression of Xbp1-eGFP in Drosophila heads. α-Tubulin was used as a loading control. (E) The normalised amount of the Xbp1-eGFP band in the HTorAΔE expressing brains was 3.68-fold higher than that in the HTorAWT-expressing brains.

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