Table 1 Overview of the five MinION® runs
From: Genome assembly using Nanopore-guided long and error-free DNA reads
Run1 | Run2 | Run3 | Run4 | Run5 | |
|---|---|---|---|---|---|
DNA library | 1 | 2 | 3 | 4 | 4 |
DNA fragment size | 8 kb | 20 kb | 20 kb | 20 kb | 20 kb |
Flowcell chemistry | R7 | R7 | R7.3 | R7.3 | R7.3 |
Number of reads | 9,241 | 3,990 | 6,052 | 11,957 | 35,252 |
Cumulative size (Mb) | 21.4 | 19.3 | 40.8 | 34.5 | 88.9 |
N50 size (bp) | 5,388 | 11,288 | 10,217 | 12,729 | 13,967 |
Average size (bp) | 2,314 | 4,830 | 6,746 | 2,886 | 2,523 |
% of 2D reads | 6.5% | 13.6% | 43.3% | 11.6% | 9.7% |
% of 2D bases | 14.6% | 27.1% | 57.1% | 42.7% | 44.6% |