Table 2 Strains and plasmids used in this study
From: Characterization of chromosomal and megaplasmid partitioning loci in Thermus thermophilus HB27
Name | Description | Source/reference |
|---|---|---|
Plasmids | Â | Â |
pUC18 | high-copy-number cloning vector | [68] |
pMK18 | E. coli/T. thermophilus shuttle vector, Tth (repA), Ec (oriE), KmR | [63] |
pUC-ΔparABc::kat | allele exchange vector for generating ΔparABc, ori pUC, KmR | this study |
pUC-ΔparABm::blm | allele exchange vector for generating ΔparABm, ori pUC, BlmR | this study |
pUC-ΔparAmN-1 | allele exchange vector for generating ΔparAmN-1, ori pUC, BlmR | this study |
pUC-ΔparAmN-2 | allele exchange vector for generating ΔparAmN-2, ori pUC, BlmR | this study |
pUC-ΔparBm::blm | allele exchange vector for generating ΔparBm, ori pUC, BlmR | this study |
pMK-parAm | pMK18 derived vector, allowing overexpression of ParAm in Tth | this study |
pMK-parBm | pMK18 derived vector, allowing overexpression of ParBm in Tth | this study |
pMK-parABm | pMK18 derived vector, allowing overexpression of ParABm in Tth | this study |
pET21a | expression vector, PT7, lacI, pBR322 ori, AmpR | Novagen, Germany |
pET21a-parBc | pET21a derived vector, allowing overexpression of ParBc in Ec | this study |
pET21a-parBm | pET21a derived vector, allowing overexpression of ParBm in Ec | this study |
pMK-sgfp | pMK18 derived vector, allowing expression of sGFP in Ec and Tth | this study |
pMKparBc-sgfp | pMK18 derived vector, allowing expression of parBc-sGFP in Ec and Tth | this study |
pMKparBm-sgfp | pMK18 derived vector, allowing expression of parBm-sGFP in Ec and Tth | this study |
T. thermophilus strains | Description | Source/reference |
HB27 | Thermus thermophilus | DSM 7039 |
HB27Δbgl | deletion of ORF TT_P0042 in HB27 | [69] |
TL-1 | carotenoid synthesis deficient, otherwise is considered as wild type | this study |
ΔparABc | HB27 derivative with parABc replaced by kat | this study |
ΔparAmN-1 | HB27 derivative with the N-terminal region of parAm replaced by blm in parABm transcription opposite direction | this study |
ΔparAmN-2 | HB27 derivative with the N-terminal region of parAm replaced by blm in parABm transcription co-linear direction | this study |
HB27/ParABm | HB27 derivative carrying a plasmid-borne copy of parABm | this study |
ΔparAmN-1/ParABm | ΔparAmN-1 derivative carrying a plasmid-borne copy of parABm | this study |
TMP0 | HB27 derivative carrying pMK18 vector | this study |
TMP01 | HB27 derivative permitting overexpression of ParAm | this study |
TMP02 | HB27 derivative permitting overexpression of ParBm | this study |
TL-1/parBc-sGFP | TL-1 derivative permitting expression of ParBc-sGFP | this study |
TL-1/ParBm-sGFP | TL-1 derivative permitting expression of ParBm-sGFP | this study |
ΔparAmN-1/ParBm-sGFP | ΔparAmN-1 derivative permitting expression of ParBm-sGFP | this study |