Table 3 Oligonucleotides used in this study for PCR genotyping and DNA sequencing
Primer name | 5′-3′ sequence |
|---|---|
PCR genotyping | |
Pxyr1-ver-F | AAGGATGCCGACTTAACGAAC |
Txyr1-ver-R | AGTCGCTCATGATCCTACCAG |
Xyr1-ver-R | CCTGGCAGCAATAAGAGAGC |
Xyr1-ver-F | CCTTGCGGATAAGTGGGATC |
5Pxyr1-ver-F | CCAGCTGCCACTCTCATG |
GFP-ver-R | AAGCACTGCACGCCGTA |
GFP-ver-F | TACGGCGTGCAGTGCTTC |
Pgpdh-ver-F | TGCTAAGGTACCTAGGGAGGGA |
hph-ver-R | CAAGCACTTCCGGAATCG |
act-mp-F | ACTTTCGGCCGCATTCTG |
act-mp-R | AGCCAGGATCTTCATCAGGTAG |
DNA sequencing | |
Pxyr1-seq-F | GACAGCAGCAGTAGTCAGGT |
Txyr1-seq-F | TTGCTGAAAGTGAAGAGGG |
xyr1-seq-F | CCGTCTCCCAAGACTAGC |
5′xyr1-seq-F | CACATTGAGGGCTCTGTC |
3′xyr1-seq-F | AAGCATCTGCCATTGTCC |
Xyr1∆A2294-seq-F | GCCACGATGCAGGGAA |
3′GFP-seq-F | CCGACAACCACTACCTGA |
5′GFP-seq-F | GCCACAAGTTCAGCGTG |
Pgpdh-seq-F | CATGCAGTTGCTAAGGTACC |
Tgpdh-seq-R | GACTGTGAGCATGTGATGGC |