Figure 5

bpa-miR-5364 interacts with predicted target gene 3′UTRs. Graph shows the mean ratio +/− standard deviation of 5–6 replicate wells comparing Firefly to Renilla luciferase signal in the presence of miR-5364 and a synthetic gene containing five repeats of a perfect miR-5364 binding site or the 3′UTRs of predicted target mRNAs, Bm1_05425, Bm1_27305 and Bm1_25620. Ratios using miR-5364 cloned in the forward orientation (miRNA F) and in reverse orientation (miRNA R) are indicated. Percentage reduction in signal was calculated by comparison of the two. Figures show data from one of three representative experiments, except for plasmid Bm1_25620 which was repeated twice with comparable results.