Figure 6From: A novel member of the let-7 microRNA family is associated with developmental transitions in filarial nematode parasitesUptake of fluorescently labelled oligonucleotides from culture medium by B. pahangi. Uptake of small RNAs from media was assessed after 24–48 hours of culture using 0.5 μM Cy3-labelled siRNA. Panels A-D show mosquito-derived L3 after 48 hours (scale bar represents 100 μm in A&B and 25 μm in C&D). Panels E & F show uptake in day 5 p.i. L3 after 24 hours (scale bar 25 μm). No fluorescence was observed in controls cultured in the absence of Cy3-labelled siRNA (not shown).Back to article page