Fig. 5

HSFY copy number estimates. Estimated copy number of HSFY in suid species measured relative to SRY. Two SRY copies are present in S. scrofa; SRY copy numbers in other species are unknown, hence estimates are shown for one and two copies. In both cases, there is a clear difference between high HSFY copy number species and low copy number species; this can be explained by two separate amplifications - one in the P. larvatus lineage, and one in the Sus lineage. Note that estimates assume 100 % PCR efficiency and equal signal from all amplicons, and cannot detect any copies with variation at the primer binding sites. Full data is given in Table 1