Fig. 1

Study design, developmental outcome and differential expression datasets of YD vs. SP embryos. a Study design. Transcriptome profiles were generated at 4 time-points until 48 hpf. The images represent YD and SP embryos at the given time points. YD embryos show a reduced yolk volume (stained by ORO and marked within dashed line) and size. b Survival of YD (25 % yolk reduction) and SP embryos during one week post-fertilization in YD (treated) and SP (control-punctured) embryos during 7 days after treatment is >90 % in each group. c Average somite number from 11 hpf to 15 hpf in YD (24 % yolk reduction) and SP embryos. d Gender of adult fish, number of fish per treatment noted inside bars. A paired T-test for the three batches shows a significant increase in females. e Box-plot of differential expression (DE) levels at each time-point for genes that were significantly differentially expressed (DE genes). f Number of DE genes obtained at each time-point. SP: down-regulated in YD; YD: up-regulated in YD; ALL: all genes which passes expression level threshold and were included in this study (see methods for details); Ens: genes from microarray data with Ensembl identifier. Union indicates union of genes with Ensembl identifier