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Table 1 Proteins whose expression was significantly induced (+) in XZ5 leaves but down-regulated (-) /unchanged in XZ54, or unchanged in XZ5 but down-regulated in XZ45 after 9 and 20 day drought stress

From: Comparative proteomic analysis of drought tolerance in the two contrasting Tibetan wild genotypes and cultivated genotype

Spot No.

Protein name

C. I. (%)

Accession number

MW (Da)

pI

AASC (%)

Fold increase (+) or decrease (-)

Function

XZ5

XZ54

ZAU3

 

Nine day drought stress (SMC 10 %)

A1

Ribulosebisphosphate carboxylase small chain clone 512 [Triticum aestivum]

100

gi|132107

13275

5.8

35.0

+2.8

−2.2

−0.2

Photosynthesis

A2

Adenosylhomocysteinase [Nicotiana tabacum]

99.9

P68173|SAHH_TOBAC

53070

5.5

23.3

+1.9

−2.4

−1.9

Amino-acid biosynthesis

A3 (C2)a

Melanoma-associated antigen p97 [Gallus gallus]

96.2

gi|45383930

80860

5.9

22.0

+1.7

NE

−106

Stress response

A4 (B1 C3)

Type I chlorophyll a/b-binding protein b [Amaranthus tricolor]

100

gi|13676406

16608

4.6

18.8

+5.6

NE

NE

Photosynthesis

A5

Unnamed protein product

100

gi|74190672

41738

5.4

37.6

+2.8

−0.1

+0.003

Unknown

A6

Glutathione S-transferase 1 [T. aestivum]

100

P30110|GSTF1_WHEAT

25811

5.3

29.3

+0.1

NE

−1.7

Stress response

A7

Ribulosebisphosphate carboxylase large chain (RuBisCO large subunit) [Welwitschia mirabilis]

99.2

RBL_WELMI

49579

6.3

25.1

+0.5

NE

+2.2

Photosynthesis

A8

Ribulosebisphosphate carboxylase large chain Precursor [Saccharum hybrid]

99.9

Q6L391|RBL_SACHY

52695

6.3

21.6

+4.5

−2.1

−106

Photosynthesis

A9

Ribulosebisphosphate carboxylase large chain precursor [T. aestivum]

100

RBL_WHEAT

53445

6.2

34.8

+3.0

+0.1

−3.0

Photosynthesis

A10 (D3)

Ribulosebisphosphate carboxylase large chain precursor [Pisum sativum]

98.8

P04717|RBL_PEA

52730

6.6

25.5

+11.4

−2.6

−1.8

Photosynthesis

A11 (C9 D4)

FTSH1 (FtsH protease 1); ATP-dependent peptidase/ATPase/ metallopeptidase [Arabidopsis thaliana]

100

gi|18402995

76712

5.6

19.0

+1.3

−2.5

1.4

Stress response

 

Twenty day drought stress (SMC 4 %)

B1 (A4 C3)

Type I chlorophyll a/b-binding protein b [Amaranthus tricolor]

100

gi|13676406

16608

4.6

18.8

+106

NE

NE

Photosynthesis

B2 (A14)

Transketolase, chloroplast [Zea mays]

99.9

Q7SIC9|TKTC_MAIZE

72948

5.5

15.1

+1.5

NE

NE

Photosynthesis

B3

ATP synthase beta subunit [Catabrosa aquatica]

100

gi|110915610

49371

5.1

53.5

+2.5

−2.1

+2.8

Energy

  1. AASC, Amino acid sequence coverage; Protein spot ID refers to numbers in Fig. 1a-b. Accession number of top database match from the NCBInr database. Fold increase and decrease were calculated as drought/control, and –control/drought for up and down -regulated proteins, respectively. All ratios shown are statistically significant (p < 0.05). +106 and -106 referred to the specific expressed and totally inhibited proteins, respectively. NE, Non Expression. SMC, soil moisture content. athe same protein spots identified at different stages