Fig. 3

Phylogenetic relationship and gene conservation of Microbotryum lychnidis-dioicae and 18 compared fungi. Phylogeny (left panel) is based on concatenated MUSCLE alignments of 80 single copy genes. Species phylogeny was inferred using RAxML (PROTCATWAG model) with 1,000 bootstrap replicates; all nodes were supported by at least 99 % of replicates. Ortholog conservation (right panel) highlights genes conserved in all species (core, green), genes conserved in at least two species (conserved, blue), genes unique to the Pucciniomycotina or Microbotryomycetes (orange and red, respectively) and genes unique to a given species (species-specific, yellow)