Fig. 6

The B. pseudomallei high biofilm producer, UM5, also results in faster killing kinetics and over-expression of biofilm-associated genes compared to a second low biofilm producing isolate, UM2. a Biofilm index of UM2 and UM5. b qRT-PCR analysis of B. pseudomallei genes from seven functional categories differentially expressed by RNA-Seq analysis. The results are from a representative of three reproducible independent experiments. c Killing assay of C. elegans infected with UM2 (black line, closed square), UM5 (black line, closed triangle) and B. thailandensis (black line, open square). The graph shows the mean ± SD of three replicates (30 worms/replicate) from a representative of two independent experiments. d Mice (n = 5) were challenged intraperitonealy with a lethal dose of B. pseudomallei UM5 (triangle) or UM2 (square) and their survival was monitored. Mice challenged with UM5 succumbed to disease significantly faster (within 24 h) than those challenged with UM2 [Logrank (Mantel-Cox) test, p-value = 0.0084]