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Table 5 Effects of glucose, citric acid, and fumaric acid on the expressions of matrix production relevant genes (epsD and tapA) of SQR9 under biofilm formation conditions

From: Whole transcriptomic analysis of the plant-beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 during enhanced biofilm formation regulated by maize root exudates

Incubation time

Treatment

Fold change in expression

epsD

tapA

24-h

Glucose (500 Î¼M)

-1.82 ± 0.22

-3.62 ± 0.63*

 

Citric acid (50 Î¼M)

-1.20 ± 0.14

1.03 ± 0.08

 

Fumaric acid (50 Î¼M)

2.97 ± 0.55*

5.15 ± 0.42*

48-h

Glucose (500 Î¼M)

-1.13 ± 0.13

-1.18 ± 0.10

 

Citric acid (50 Î¼M)

1.48 ± 0.21

2.27 ± 0.17*

 

Fumaric acid (50 Î¼M)

1.58 ± 0.30

1.31 ± 0.10

  1. The fold changes revealed by real-time PCR of the selected genes were determined based on the threshold cycle (Ct) values and 2-△△Ct method (94). Three replicates were performed for each gene. The asterisks represent a gene expression levels with fold changes > 2
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BMC Genomics

ISSN: 1471-2164

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