Fig. 1

Quality metrics show the efficacy of the AbaSI enzyme, sufficient depth of sequencing, and suitability of sample. a The AbaSI enzyme is said to cut 11-13 bp downstream of a glucosylated 5hmC. An example from a sequencing quality control plot showed a peak of cytosine and guanine bases at the 11th and 12th positions of sequencing reads, respectively. b No correlation was observed between the number of uniquely mapped AbaSI-Seq reads and the number of 5hmC detected per subject, thereby showing sufficient depth of sequencing. c-d No correlation was observed between either the subjects’ ages (c) or their PMIs (d) and the total number of 5hmC sites detected