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Fig. 5 | BMC Genomics

Fig. 5

From: Identification of candidate gonadal sex differentiation genes in the chicken embryo using RNA-seq

Fig. 5

Novel candidate female genes are translated in the embryonic gonads. Immunofluorescence staining using antibodies raised in house (CAPN5 and GPR56) or commercial antibodies (FGFR3) in E8 gonads. CAPN5 protein is only weakly expressed in males (a), but shows high expression in a subset of cells in the female gonads (b). These cells are present in the juxta-cortical medulla, and staining appears cytoplasmic (c). GPR56 is expressed in the cortex of the female (e, f), where it appears cytoplasmic, although this staining is weak, and GPR56 is weak in male gonads (d). FGFR3 is expressed in the male gonads (g) and in the female gonads (h), where it is strongly expressed in the membrane or cytosol of the germ cells (see insert Gi). Strong staining in the female cortex (i) is probably due to germ cell expression, although may also be due to additional cortical cells expressing this receptor

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