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Fig. 7 | BMC Genomics

Fig. 7

From: Genome analysis and in vivo virulence of porcine extraintestinal pathogenic Escherichia coli strain PCN033

Fig. 7

Phenotype analysis of PCN033 and PCN061. a Transmission electron micrographs of PCN033 and PCN061 strains. Bacteria were grown overnight at 37 °C in solid medium (TSA). The scale bar indicates the manification size. The scale bars in micrographs of PCN033 were 1000 nm, 500 nm and 200 nm, successively (from left to right). The scale bars in micrographs of PCN061 were 2000 nm, 500 nm and 200 nm, successively (from left to right). Thin arrows indicate fimbriae, thick arrow indicats flagella. b Biofilm formation of PCN033 and PCN061 strains on the polystyrene surface. Each strain was tested in three-wells in a 96-well microtiter plate. The optical density of the bacterial biofilm formation were recorded at OD600nm after 24 h incubation. Data are expressed as relative biofilm values (mean ± standard deviation) and representative of three independent experiments. The broken line indicates the cutoff value (ODc = 0.192) for determining a biofilm producer, defined as two times the negative control value. * P < 0.05. c Swarming motility assay. Assays were performed in triplicate at 37 °C

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