Fig. 4

Quantitative RT-PCR analyses of glutamine-responsive transcription factor genes. Seventeen-day-old rice seedlings grown in hydroponic solution without nitrogen were subsequently transferred to medium containing 2.5 mM glutamine, glutamate, or 1.43 mM ammonium nitrate for 0, 15′, 30′, 1, 4, and 24 h. Total RNA extracted from roots was used for quantitative RT-PCR to analyze the expression of ZOS5-02 (a), LBD37-like Os07g0589000 (b), LBD37-like Os03g0445700 (c), AP2/ERF106 (d), bHLH Os04g0301500 (e), IRO2 (f), DREB1A (g), MYB-like Os07g0119300 (h), NAC5 (i), and WRKY69 (j). The expression level of each gene in the control sample (0 h) was set at 1. Fold change indicates the relative expression of each gene as compared to that of control. Quantitative RT-PCRs were performed in triplicate for each sample in three independent experiments. All of the quantifications were normalized to the nuclear gene UBC3 (Os02g0634800)