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Table 2 Amino acid contents (nmole/g FW) of 17-day-old rice seedlings

From: Glutamine rapidly induces the expression of key transcription factor genes involved in nitrogen and stress responses in rice roots

 

Roots

Shoots

− N

+ Gln

+ Gln/ − N (fold change)

− N

+ Gln

+ Gln/ − N (fold change)

Glu

550.9 ± 52.9

669.3 ± 83.8

1.22 ± 0.17

1243.9 ± 280.7

1126.5 ± 205.5

0.93 ± 0.21

Asp

252.7 ± 39.0

350.8 ± 51.8

1.40 ± 0.21*

281.0 ± 60.3

228.0 ± 66.0

0.82 ± 0.18

Ser

171.7 ± 22.3

181.4 ± 17.2

1.06 ± 0.10

874.7 ± 224.9

666.0 ± 164.1

0.77 ± 0.16

Gln

108.1 ± 15.0

1275.5 ± 397.8

11.62 ± 2.36*

622.9 ± 302.7

469.0 ± 262.3

0.75 ± 0.21

Ala

81.1 ± 11.7

142.9 ± 24.7

1.76 ± 0.21*

1063.5 ± 297.9

847.3 ± 183.3

0.81 ± 0.11

Val

46.5 ± 6.8

47.9 ± 5.5

1.04 ± 0.15

225.7 ± 64.7

160.4 ± 38.3

0.73 ± 0.12

Cys

44.9 ± 11.0

46.8 ± 4.7

1.08 ± 0.19

340.7 ± 96.2

273.1 ± 81.3

0.80 ± 0.07

Leu

36.9 ± 6.9

35.2 ± 4.4

0.98 ± 0.22

60.4 ± 13.0

44.1 ± 9.0

0.74 ± 0.08

Thr

36.2 ± 2.1

39.1 ± 4.1

1.08 ± 0.13

160.0 ± 44.3

120.9 ± 34.7

0.76 ± 0.13

Pro

30.8 ± 13.5

28.4 ± 9.2

0.99 ± 0.30

166.4 ± 83.1

139.4 ± 79.8

0.82 ± 0.09

Ile

28.1 ± 4.5

25.5 ± 2.9

0.93 ± 0.17

49.8 ± 17.9

32.4 ± 7.2

0.68 ± 0.12

Trp

26.8 ± 9.6

26.5 ± 9.6

0.99 ± 0.06

416.7 ± 286.2

297.7 ± 144.1

0.78 ± 0.14

Lys

26.7 ± 5.1

27.4 ± 3.6

1.05 ± 0.19

183.0 ± 144.5

136.1 ± 95.7

0.80 ± 0.10

Tyr

24.9 ± 4.5

29.1 ± 2.0

1.19 ± 0.15

333.9 ± 87.0

264.9 ± 72.7

0.81 ± 0.19

His

24.0 ± 10.6

20.5 ± 5.6

0.91 ± 0.17

609.1 ± 488.3

396.3 ± 289.7

0.68 ± 0.14

Asn

21.6 ± 11.4

21.5 ± 11.2

1.03 ± 0.40

130.7 ± 95.3

119.9 ± 84.8

0.96 ± 0.32

Arg

20.2 ± 4.3

14.8 ± 3.5

0.74 ± 0.14

60.9 ± 16.6

43.6 ± 11.4

0.72 ± 0.12

Phe

14.8 ± 2.4

15.7 ± 3.6

1.06 ± 0.16

378.6 ± 96.2

291.5 ± 61.8

0.78 ± 0.09

Gly

14.6 ± 1.2

14.1 ± 1.9

0.96 ± 0.07

87.6 ± 25.3

67.6 ± 17.8

0.80 ± 0.21

Met

9.0 ± 0.7

10.4 ± 1.0

1.16 ± 0.11

60.4 ± 13.1

44.5 ± 7.5

0.74 ± 0.05

  1. − N without nitrogen, + Gln treated with 2.5 mM glutamine for 30 min. Results were derived from four biological replicates. *indicates p < 0.05 after analysis with Student’s t-test