Fig. 4

pdm locus enhancers direct gene expression during multiple developmental stages. a The nub-53 enhancer activates expression in stage 10 ventral nerve cord (VNC) cells. During postembryonic neurogenesis, nub-53 cis-regulatory activity in the larval brain lobes and anterior VNC was detected, as well as in the adult optic lobe and central brain. b nub-54 regulates expression in putative embryonic cardiac cells, larval brain lobes, and putative adult nodular neurons and median neurosecretory cells. c pdm2-15 enhancer activity in putative embryonic procephalon and VNC cells was detected at stage 13. pdm2-15 also directs expression in larval anteromedial and posterolateral VNC neurons and in the adult lateral horn. d pdm-37a activates expression in the embryonic clypeolabrum, salivary gland, and subesophageal ganglion, in larval NB lineages and throughout the adult brain