Fig. 5

Enhancer-reporter transgene analysis reveals multiple embryonic neural-specific enhancers. Shown are 8 of the 41 embryonic enhancers that were identified in this study (see Additional file 4: Table S1 for additional embryonic enhancer descriptions). a–h Whole-mount Gal4 mRNA in situ hybridizations (ventral, lateral, and dorsal views); anterior up. a At stage 13, nub-12 directs expression in a subset of VNC and cephalic lobe neurons. b nub-41 regulates expression in a subgroup of lateral cephalic lobe NBs during stage 11. c In stage 9 embryos, nub-44 directs reporter expression in a subset of lateral cephalic lobe NBs. d nub-46 regulates reporter expression during stage 11 of NB lineage development in the VNC and cephalic lobes e pdm2-7b activates reporter expression in a limited subgroup of cephalic lobe NB during stage 10. f In stage 13 embryos, pdm2-25 also regulates expression in a small subset of cephalic lobe NBs. g pdm2-31a activates expression in cephalic lobe NBs at stage 12. h In stage 11 embryos, pdm2-34 directs reporter expression in subsets of VNC and cephalic lobe NBs. To definitively identify neuronal cell types, further work using neuronal-specific lineage markers is required