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Fig. 3 | BMC Genomics

Fig. 3

From: Early transcriptional events linked to induction of diapause revealed by RNAseq in larvae of drosophilid fly, Chymomyza costata

Fig. 3

Results of three-step validation of RNAseq differential gene expression analysis. Each heat map (a) shows log2 fold transformed differences in mRNA relative abundances in 25 selected genes. The columns show (from left to right): original results obtained by DeSEQ2 analysis of RNAseq (Illumina) sequencing data; direct validation by qRT-PCR (using the same total RNA samples as used for RNAseq analysis); qRT-PCR analysis conducted on an independent replication of the experiment using wild-type and NPD-mutant Chymomyza costata larvae. The linear regression analysis (b) shows good correlation between RNAseq results and direct qRT-PCR validation. The larvae were exposed to Long Day (LD), Short Day (SD) and Transfer (T) from LD to SD photoperiodic conditions (see Fig. 5 for schematic depiction)

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