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Table 1 The neonicotinoid response of the susceptible S-GR6 and the resistant B. tabaci laboratory strains. Toxicological data to the neonicotinoids imidacloprid, acetamiprid and thiacloprid and toxicity of imidacloprid after pre-exposure to synergist piperonyl butoxide (PBO)

From: Transcription analysis of neonicotinoid resistance in Mediterranean (MED) populations of B. tabaci reveal novel cytochrome P450s, but no nAChR mutations associated with the phenotype

Strain

n

LC50 (95 % CI)

RR (95 % CI)

SR (95 % CI)

IMIDACLOPRID

 S-GR6

580

7.5 (5.4–10)

-

 

 S-GR6 + PBO

543

6.0 (4.3–7.1)

-

-

 GR4_AP

586

69 (46–98)

9 (6–13)

 

 GR4_AS

479

423 (258–657)

56 (36–87)

 

 GR4_AS + PBO

433

185 (67–302)

30 (17–38)

1.9 (1.7–2.3)

 GR9_IP

547

176 (82–268)

23 (15–35)

 

 GR9_IS

479

301 (186–443)

40 (28–58)

 

 GR9_IS + PBO

435

207 (164–332)

34 (25–50)

1.2 (1.1–1.4)

 GR0_IP

699

82 (60–106)

11 (7–16)

 

 GR0_IS

451

177 (55–336)

24 (14–39)

 

 GR0_IS + PBO

425

52 (10–148)

9 (3–16)

2.7 (2.3–3.1

ACETAMIPRID

 S-GR6

503

7.9 (5.1–11)

-

 

 GR4_AP

442

44 (20–80)

5 (3–9)

 

 GR4_AS

539

157 (99–242)

20 (15–33)

 

 GR9_IP

391

56 (28–89)

7 (4.1–11)

 

 GR9_IS

535

137 (110–163)

17 (11–26)

 

 GR0_IP

402

33 (25–59)

4 (2.8–6.3)

 

 GR0_IS

460

71 (42–105)

9 (5.3–15)

 

THIACLOPRID

 S-GR6

517

11 (4.2–22)

-

 

 GR4_AP

422

>30,000

>3,000

 

 GR4_AS

300

9,740 (835–27,145)

924 (378–2,259)

 

 GR9_IP

449

>30,000

>3,000

 

 GR9_IS

320

13,061 (5,253–25,522)

1,239 (720–2,134)

 

 GR0_IP

422

>30,000

>3,000

 

 GR0_IS

281

10,477 (3,373–21,100)

994 (457–2,161)

 
  1. RR resistance ratio at LC50, the Lethal Concentration of 50 %, CI confidence intervals resistance characteristics, SR synergist ratio: RR/RR after pretreatment with PBO