Fig. 5

a Impact of no. of PCR cycles on the forward MS error rate. Increasing number of cycles did increase forward error rate with marginal significance for the FG library preparation method with Q5 Taq (t-test 15 cycles [mean 0.58 %] vs 25 cycles [mean 0.64 %] p=0.11). b Impact of PCR starting amount on percentage of chimeric reads. Decreased starting amount reduced percentage of chimeras for the FG library preparation method with HiFi Taq but not significantly (t-test comparing 1 ng [mean 0.08 %] and 10 ng [mean 0.2 %] p=0.20). c Impact of no. of PCR cycles on the percentage of chimeric reads. Increasing cycle number increased the percentage of chimeric reads for the FG library preparation method with Q5 Taq (t-test 15 cycles [mean 0.00 %] vs 25 cycles [mean 0.66 %] p=0.0245)