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Fig. 6 | BMC Genomics

Fig. 6

From: Epigenetic modification with trichostatin A does not correct specific errors of somatic cell nuclear transfer at the transcriptomic level; highlighting the non-random nature of oocyte-mediated reprogramming errors

Fig. 6

Epigenetic reprogramming and chromatin remodeling in reconstituted oocytes. a1-a’5 Stepwise pattern of 5mC at different intervals (0, 4, 8, 12, 24 hpr) in CTR-NT (a1-a5) and TSA-NT (a’1-a’5). b Comparison between intensity levels of 5mC at different intervals (0, 4, 8, 12, 24 hpr) in CTR-NT (blue line) and TSA-NT (red line). c1-c’1 Stepwise pattern of 5hmC at different intervals (0, 4, 8, 12, 24 hpr) in CTR-NT (c1-c5) and TSA-NT (c’1-c’5). d Comparison between intensity levels of 5hmC at different intervals (0, 4, 8, 12, 24 hpr) in CTR-NT (blue line) and TSA-NT (red line). e1-e5 Stepwise pattern of nuclear remodeling pictured from 0.5 to 24 h post reconstitution. The merged images of microtubule and nuclear immunostaining have been shown. e1 the very sign of nuclear swelling without spindle formation, e2 nuclear remodeling without spindle formation, e3 premature chromosome condensation with developing spindles, e4 single pronucleus completely developed with an early SCNT zygote,) first mitotic division of the newly developed SCNT zygote. Bars with different letters differ significantly at P < 0.05. Scale bar represents 100 μm

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BMC Genomics

ISSN: 1471-2164

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