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Fig. 5 | BMC Genomics

Fig. 5

From: Genome-wide analysis of tomato NF-Y factors and their role in fruit ripening

Fig. 5

TRV-mediated virus induced gene silencing (VIGS) of genes in tomato fruit. The inflorescence peduncles attached to the fruit were injected with Agrobacterium tumefaciens transformed with TRV alone, or with pTRV2 carrying a fragment of a target gene. a pTRV-PDS, (b) pTRV2-Solyc08g062210/Solyc11g065700, (c) pTRV2-Solyc06g069310, (d) pTRV2-Solyc07g065500, (e) pTRV2-Solyc01g087240. Silencing of the target gene led to a decrease in gene expression, and different fruit color phenotypes compared with plants that were transformed with the empty vector control, which displayed normal ripening fruits (a-e). RNA was extracted from the control and the red and yellow areas of the gene-silenced tomato fruits. After reverse transcription, both the control and silenced samples were assayed for the presence of the TRV virus using PCR with primers specific to the TRV2 vector (a, c), and a virus coat protein (CP) gene (b, d). Lane 1 = negative control, Lane 2 = red part of the silenced tomato fruit, lane 3 = yellow part of the silenced tomato fruit, and lane 4 = the positive control. RT-PCR analysis of the target genes in the silenced tomato fruits showed significantly different expression in the yellow areas of the fruits. Asterisks indicate a significant difference as determined by Student’s t-test (* = p < 0.05, ** = p < 0.01)

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