Identification of miRNAs and their targets by high-throughput sequencing and degradome analysis in cytoplasmic male-sterile line NJCMS1A and its maintainer NJCMS1B of soybean

Table 3 Identification of new miRNA members of known miRNA families

Index	miRNA_name	miRNA_sequence	Length (nt)	MFE (kcal/mol/nt)	MFEI	NJCMS1A_ count	NJCMS1B_ count	3p/5p^a)
1	N-gma-miR164l	TGGAGAAGGGGAGCACGTGCA	21	−0.53	0.98	12	8	5p
2	N-gma-miR164m	TGGAGAAGGGGAGCACGTGCA	21	−0.52	0.97	12	8	5p
3	N-gma-miR164n	TGGAGAAGGGGAGCACGTGCA	21	−0.54	0.97	12	8	5p
4	N-gma-miR164o	TGGAGAAGGGGAGCACGTGCA	21	−0.53	1.15	12	8	5p
5	N-gma-miR164p	TGGAGAAGGGGAGCACGTGCA	21	−0.48	0.90	12	8	5p
6	N-gma-miR164q	TGGAGAAGGGGAGCACGTGCA	21	−0.49	1.07	12	8	5p
7	N-gma-miR164r-5p	TGGAGAAGCAGGGCACATGCT	21	−0.57	1.20	78	35	5p
8	N-gma-miR164r-3p	CTTGTGTCCTACTTCTCCAGC	21	−0.57	1.20	4	0	5p
9	N-gma-miR156ac	CTGACAGAAGATAGAGAGCAC	21	−0.40	0.86	85	69	5p
10	N-gma-miR395n	CTGAAGTGTTTGGGGGAGCTT	21	−0.41	0.91	5	6	3p
11	N-gma-miR399i-5p	GAGCAATTCTCCTTTGGCAGA	21	−0.48	1.15	2	0	5p
12	N-gma-miR399i-3p	TGCCAAAGGAGAATTGTCCTG	21	−0.48	1.15	6	0	3p
13	N-gma-miR399j	TGCCAAAGGAGATTTGCCCTG	21	−0.44	0.98	0	9	3p
14	N-gma-miR399k-5p	GAGCAAATCTCCATTGGCAGT	21	−0.45	1.01	0	5	5p
15	N-gma-miR399k-3p	TGCCAAAGGAGATTTGCCCTG	21	−0.45	1.01	0	9	3p
16	N-gma-miR399l	TGCCAAAGGAGAGCTGCCCTG	21	−0.43	1.07	97	53	3p
17	N-gma-miR399m	TGCCAAAGGAGAGCTGCCCTG	21	−0.45	1.17	97	53	3p
18	N-gma-miR530f	TGCATTTGCACCTGCGCTTTG	21	−0.47	0.96	8	0	5p
19	N-gma-miR4380c	TGGTTCATACGGATTGTTGAT	21	−0.47	1.12	5	0	3p
20	N-gma-miR4380d	TGGTTCATACGGATTGTTGAT	21	−0.33	1.28	5	0	3p
21	N-gma-miR4348d	TGTCAAACTTGCAAGATGATA	21	−0.45	1.46	0	10	5p
22	N-gma-miR5673b	TGGAATCTCGCGGAAGACATC	21	−0.61	1.27	5	0	3p
23	N-gma-miR5786b	CGTCGCAGGATAGAGGGCACTG	22	−0.39	0.94	0	8	5p

ISSN: 1471-2164