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Fig. 2 | BMC Genomics

Fig. 2

From: Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome

Fig. 2

L-Leu increased translation of genes with poor translational efficiency in RBS cells. The corrected (cor) and RBS mutant (mut) cells were treated with either D-Leu or L-Leu for 3 h or 24 h. Cells were collected for ribosome profiling. a The boxplot shows the translational efficiency for genes with a 5’ TOP sequence. These mRNAs showed poor translational efficiency in RBS cells, which was partly rescued by L-Leu treatment. Corrected cells with D-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 6.9e-22; Mutant cells with L-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 4.4e-14; Corrected cells with D-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 8.8e-16; Mutant cells with L-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 1.9e-16. b The boxplot shows the translational efficiency for genes with a PRTE sequence. These mRNAs showed poor translational efficiency in RBS cells that was partially improved by L-Leu. Corrected cells with D-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 7.1e-14; Mutant cells with L-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 8.5e-9; Corrected cells with D-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 2.8e-14; Mutant cells with L-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 5.3e-15. c The boxplot shows the translational efficiency for genes previously defined to be hypersensitive to mTOR inhibition via Babel analysis [28]. These mRNAs showed poor translational efficiency in RBS cells that was partially improved by L-Leu. Corrected cells with D-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 0.0002; Mutant cells with L-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 0.14; Corrected cells with D-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 3.5e-8; Mutant cells with L-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 2.1e-6

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