Fig. 3

L-Leu treatment partially rescued mitochondrial function in RBS cells. a The heatmap shows that genes with mitochondrial function are differentially expressed in RBS cells. The yellow and purple bars indicate gene groups that are downregulated or upregulated, respectively, in the mutant cells, and are unresponsive to leucine. The subgroup that is affected by L-leucine treatment in the RBS cells is indicated by the green bar. See Additional file 2: Table S9 for GO terms for the leucine responsive cluster. b Intracellular ATP concentration was low in RBS cells but significantly improved by L-Leu treatment for 24 h. c Cytochrome c oxidase activity was impaired in RBS cells but significantly improved by L-Leu supplement for 24 h. For b and c error bars represent standard deviation of three biological replicates and the p value was calculated from a t-test