Fig. 4

High levels of snoRNAs in RBS cells were partially reduced by L-Leu treatment. We selected a group of 379 snoRNA genes based on the biotype “snoRNA” and having the words “C/D box” or “H/ACA box” in the description field from ensembl. a The boxplot shows the expression of these genes is increased in RBS cells, but partly reduced with L-Leu treatment (gene data in Additional file 2: Table S11). Corrected cells with D-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 1.4e-275; Mutant cells with L-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 3e-53; Corrected cells with D-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 7.6e-243; Mutant cells with L-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 7.1e-98. P values in a and c were generated using a t test. b The scatter plot depicts the log2 fold change for snoRNAs in RBS mutant vs corrected at 3 h D-Leu (x axis) versus the same for host genes (y axis). The correlation is 0.76. c The boxplot shows the snoRNA host gene expression was not significantly different between corrected cells and mutant cells, and the host gene expression is not affected by L-Leu treatment. Corrected cells with D-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 0.3; Mutant cells with L-Leu 24 h versus Mutant cells with D-Leu 24 h, P = 0.96; Corrected cells with D-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 0.1; Mutant cells with L-Leu 3 h versus Mutant cells with D-Leu 3 h, P = 0.62. d The scatter plot depicts the log2 fold change for snoRNAs in RBS mutant L-Leu vs D-Leu at 3 h (x axis) versus the same for host genes (y axis). The correlation is 0.07