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Fig. 4 | BMC Genomics

Fig. 4

From: Coupling high-throughput genetics with phylogenetic information reveals an epistatic interaction on the influenza A virus M segment

Fig. 4

Functional relevance of the profiling result. a At each amino acid residue, essentialness represents the fraction of profiled substitutions being deleterious. The essentialness for those residue with ≥ 2 substitutions being profiled is shown. Each data point is colored according to the value of essentialness: essentialness = 0 (blue), 0 < essentialness ≤ 0.25 (marine), 0.25 < essentialness ≤ 0.5 (white), 0.5 < essentialness ≤ 0.75 (orange), 0.75 < essentialness ≤ 1 (red). b The distributions of essentialness for individual residues on M1 and M2 are shown as boxplots. c The essentialness is projected on the structure of homodimer of M1 N-terminal domain (PDB: 1EA3) [39]. Residues are color-coded as that of panel a. Those residues with < 2 substitutions being profiled is colored in grey. d Individual residues on M1 N-terminal domain were categorized into buried residues, surface-exposed residues at the homodimer interface, and other surface-exposed residues. The distributions of essentialness for these three categories are shown as boxplots. e The essentialness is projected on the structure of homotetramer of M2 ion channel (PDB: 2RLF) [72]. Residues are color coded according to that of panel a. Those residues with < 2 substitutions being profiled is colored in grey

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