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Table 1 Verification of DEGs using real-time qRT-PCR

From: De novo transcriptome analyses of host-fungal interactions in oil palm (Elaeis guineensis Jacq.)

Unigene

Putative identity

Fold change of expression using

  

Transcriptome sequencing

Real-time qRT-PCR

  

In G-treated oil palm seedlings compared to untreated seedlings

15029

1-Aminocyclopropane-1-carboxylate oxidase

107

4.84±0.43

13913a

1-Aminocyclopropane-1-carboxylate oxidase

−2.70

1.01±0.03

21170

Chitinase

12.5

3.59±0.58

21171

Chitinase

16.8

2.93±0.84

21174

Chitinase Class III

10.1

4.03±0.91

595

Early flowering protein

5.85

3.25±0.66

23670

β-1,3-Glucanase

6.17

9.18±0.63

4320a

NBS-LRR-resistance protein

−10.1

1.88±0.16

152

Pathogenesis-related protein

20.4

4.84±0.36

23132

Pathogenesis-related protein 4

8.40

3.03±0.54

15545

Type 2 ribosome-inactivating protein precursor

9.26

2.39±0.34

15323

Thaumatin-like protein

17.5

2.29±0.16

28483

Predicted protein

2.54

2.29±0.15

28249

Hypersensitive induced reaction1

6.37

1.45±0.16

18366

Hypothetical protein SORBIDRAFT 02g042630

8.76

1.09±0.13

18282a

Fiber protein Fb2

−1.12

1.25±0.08

308

Ethylene insensitive-like protein 4

1.26

1.61±0.11

  

In T-treated oil palm seedlings compared to untreated seedlings

13913

1-Aminocyclopropane-1-carboxylate oxidase

−12.9

−1.82±0.04

21170

Chitinase

8.47

3.85±0.95

4320

NBS-LRR-resistance protein

10.4

3.21±0.48

25702

Nitrate reductase 1

9.46

2.19±0.24

  1. aGenes that show discrepancies in their gene expression patterns when measured by transcriptome sequencing and real-time qRT-PCR