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Table 1 Differentially expressed proteins detected in the liver of AA broiler chickens fed the dietary probiotic E. facecium a

From: Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus)

Spot no.

Protein name

Accession no.

Symbol ID

Mr (kDa)/pI

Sequence coverage (%)

Matched/searched

Mascot score

Av. ratio (treatment/control)

p value

Immune and antioxidant system

1

Transferrin

gi|83754919

LTF

77.49/6.70

50

75/326

1282

−1.59

7.6E − 03

2

Transferrin

gi|83754919

LTF

77.49/6.70

50

75/326

1282

−1.60

1.4E − 02

3

PIT 54 protein

gi|46395491

PIT54

52.67/4.61

23

16/252

272

−1.61

8.5E − 04

4

Serum albumin precursor

gi|45383974

ALB

71.87/5.51

76

96/411

2211

2.10

1.2E − 02

5

Serum albumin precursor

gi|45383974

ALB

71.87/5.51

80

118/411

2390

1.73

2.0E − 02

6

Serum albumin precursor

gi|45383974

ALB

71.87/5.51

80

100/411

2671

2.39

3.4E − 03

7

PIT54 protein

gi|46395491

PIT 54

52.67/4.61

23

16/252

272

−1.67

6.7E − 05

14

Fibrinogen β chain

gi|399491

FGB

53.27/7.18

46

29/222

619

−1.56

3.2E − 03

15

Serum albumin precursor

gi|45383974

ALB

71.87/5.51

31

23/263

379

1.47

1.8E − 02

16

Fibrinogen gamma chain

gi|8569623

FGG

47.49/5.40

28

19/246

296

−1.57

1.5E − 02

18

Fibrinogen gamma chain

gi|8569623

FGG

47.49/5.40

26

12/191

232

−1.70

5.8E − 04

12

Catalase

gi|53127216

CAT

60.28/8.09

39

39/290

685

2.14

8.0E − 03

13

Catalase

gi|53127216

CAT

60.28/8.09

67

68/334

1104

2.39

1.4E − 03

34

Epoxide hydrolase 2

gi|75832164

EPHX2

63.72/5.89

19

18/201

308

1.43

4.2E − 02

40

HSP108

gi|63509

HSP90B1

91.45/4.81

4

8/241

86

−1.69

1.1E − 02

46

HSP108

gi|63509

HSP90B1

91.45/4.81

3

2/241

56

−1.48

1.8E − 02

49

Annexin A6

gi|50982399

ANXA6

75.58/5.57

8

4/244

71

−1.64

2.3E − 03

50

GlutathioneS-transferase 2

gi|2981970

GSTM2

25.92/7.00

63

18/217

546

1.48

3.9E − 03

51

HSP108

gi|63509

HSP90B1

91.45/4.81

13

16/209

402

−1.76

2.4E − 02

Carbohydrate metabolism and energy production

20

α-Enolase

gi|46048768

ENO1

47.62/6.17

48

43/296

882

1.94

3.2E − 03

21

α-Enolase

gi|46048768

ENO1

47.62/6.17

38

17/227

378

1.78

4.8E − 03

23

α-Enolase

gi|46048768

ENO1

47.62/6.17

52

28/279

821

1.76

5.2E − 03

25

α-Enolase

gi|46048768

ENO1

47.62/6.17

41

39/291

880

1.77

1.3E − 02

26

α-Enolase

gi|46048768

ENO1

47.62/6.17

53

52/324

1088

1.80

6.6E − 03

27

Mitochondrial inner membrane protein

gi|57530041

IMMT

79.54/5.72

6

4/266

50

−1.74

5.4E − 03

36

α-Enolase

gi|46048768

ENO1

47.62/6.17

11

5/290

93

1.44

4.3E − 04

37

Phosphoglycolate phosphatase

gi|71894743

PGP

33.55/5.53

33

15/271

364

1.50

9.4E − 03

44

α-Enolase

gi|46048768

ENO1

47.62/6.17

8

4/296

62

−1.41

3.9E − 02

47

α-Enolase

gi|46048768

ENO1

47.62/6.17

23

11/240

313

−1.65

2.1E − 02

55

Alcohol dehydrogenase 6

gi|45384164

ADH6

40.89/7.85

16

8/231

130

3.38

2.2E − 03

56

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

gi|45382653

PCK2

71.72/8.16

11

9/196

134

1.65

7.8E − 03

58

Phosphoglycerate kinase

gi|45384486

PGK1

45.09/8.31

15

14/256

333

−1.45

1.5E − 02

Amino acid and protein metabolism

17

Alanyl-tRNA synthetase, cytoplasmic

gi|57524852

AARS

102.00/5.68

11

14/189

272

−1.47

4.5E − 03

22

Homogentisate 1,2-dioxygenase

gi|50729534

HGD

50.12/6.35

34

17/279

397

1.79

3.6E − 03

24

Betaine-homocysteine S-methyltransferase 1

gi|50755288

BHMT

45.55/7.56

52

27/265

507

1.78

3.8E − 04

29

Protein disulfide-isomerase A4

gi|57530768

PDIA4

71.29/4.96

11

7/228

115

−1.62

2.8E − 03

32

Aspartate aminotransferase, cytoplasmic

gi|809192

GOT1

46.00/8.26

54

32/245

943

1.44

4.4E − 02

33

Cystathionase

gi|118094764

CTH

44.56/6.86

38

17/233

335

1.47

4.8E − 02

41

Betaine-homocysteine S-methyltransferase

gi|50755288

BHMT

45.55/7.56

32

18/259

316

1.49

1.4E − 02

42

Prolyl-4-hydroxylase

gi|63739

P4HB

55.17/4.66

13

7/250

146

−1.89

1.1E − 03

43

Protein disulfide-isomerase A3 precursor

gi|45383890

PDIA3

56.55/5.76

28

24/238

589

−1.42

4.4E − 03

45

Elongation factor 2

gi|45382453

EEF2

96.34/6.40

6

7/255

94

−1.40

2.1E − 03

Lipid and vitamin metabolism

28

Retinal dehydrogenase 1

gi|45383031

ALDH1A1

56.40/7.49

16

14/229

111

1.41

3.7E − 02

35

3-oxo-5-β-steroid 4-dehydrogenase isoform 2

gi|118082901

AKR1D1

50.02/9.32

52

26/256

655

1.44

4.4E − 02

38

Regucalcin

gi|45382019

RGN

33.67/5.77

75

39/286

1279

1.41

2.7E − 03

48

Apolipoprotein A-I

gi|211159

APOA1

30.67/5.58

10

3/250

59

−1.42

3.6E − 02

57

Fatty acid-binding protein, liver

gi|45383728

FABP1

14.30/7.74

66

17/286

461

−1.90

2.6E − 03

Nucleotide metabolism

8

Bifunctional purine biosynthesis protein PURH

gi|28373618

ATIC

67.05/8.54

31

30/230

364

2.46

1.7E − 03

53

Dihydropyrimidinase

gi|118087274

DPYS

69.50/6.42

4

2/265

70

1.51

4.5E − 02

54

Nucleoside diphosphate kinase

gi|2827446

NME4

17.54/7.11

65

27/277

492

1.43

6.8E − 03

  1. a Spot no. corresponds to the number of protein spots in Fig. 1. Protein name is given when proteins were identified by LC-Chip ESI-QTOF MS. Accession no. is the unique number given to mark the entry of a protein in the database NCBInr. Theoretical molecular weight (Mr) and isoelectric point (pI) of the identified proteins are retrieved from the protein database of NCBInr. Sequence coverage is the ratio of the number of amino acids in every peptide that matches with the mass spectrum divided by the total number of amino acids in the protein sequence. Matched peptide is the number of paring an experimental fragmentation spectrum to a theoretical segment of protein and searched peptide is the total searched peptide. Peptides were identified from the liver of AA broiler chickens based on Mascot scores (Additional file 3: Table S3) . Mascot scores are derived from ion scores as a non-probabilistic basis for ranking protein hits. Av. ratio and p value are calculated using DeCyder software version 7.0