Fig. 5From: Discovery and profiling of small RNAs responsive to stress conditions in the plant pathogen Pectobacterium atrosepticum RT-qPCR validation of RNA-seq expression analysis. Relative expression changes of sRNAs were determined using the 2-ΔΔct method by comparing expression in starvation conditions to nutrient-rich. Error bars indicate the standard error of three independent biological replicates. Asterisks represent significant difference at p < 0.05 (Students t-test)Back to article page