Fig. 3

Translated open reading frames on the noncoding RNA Malat 1. a Distribution of RNA-seq reads (black) and ribosome footprints on the Malat1 transcript in the absence (blue) or presence of the translation inhibitors DMDA-PatA (red), harringtonine (green) and puromycin (yellow). b Same as (A) zoomed on nucleotides 200–600 of Malat1, the region with the highest pateamineA sensitive ribosome footprint density. The principal AUG ORFs are indicated: ORF1 (209–277, alternate AUG at 242), ORF 2 (270–359), ORF 3 (431–466), ORF 4 (521–550). Colors indicate different reading frames. The principal peaks of ribosome pileup are indicated with letters and are shown in detail in (c). d Effect of DMDA-PatA on ribosome footprints on the four ORFs shown in (B) and the 3’ untranslated region (nt. 1000–6983) of Malat1