Fig. 4From: High-resolution identification and abundance profiling of cassava (Manihot esculenta Crantz) microRNAsExperimental determination of miRNA cleavage of cassava mRNA targets. Total RNA from cassava leaves was subjected to 5′ RACE analysis to validate cleavage of selected predicted targets of members of the miR482/2118 superfamily. Partial mRNA sequences from target genes were aligned with corresponding miRNAs. G:U wobble pairing (circles), mismatched bases (X) and Watson-Crick pairing (vertical dashes) are indicated. Arrows indicate the cleavage sitesBack to article page