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Fig. 1 | BMC Genomics

Fig. 1

From: Post-weaning blood transcriptomic differences between Yorkshire pigs divergently selected for residual feed intake

Fig. 1

Distribution of RFI values and sample selection for RNA-seq and RT-qPCR assays. Post-weaning blood samples of 16 pigs (8 per diet) with extremely low RFI from gilts of the low RFI line (LRFI) and 16 pigs (8 per diet) with extremely high RFI from gilts of the high RFI line (HRFI) fed the high-fiber, low-energy diet (HFD) or the low-fiber, high-energy diet (LFD) were selected for RNA-seq. One sample in the LRFI-LFD group was excluded from RNA sequencing because of low quality RNA, leaving a total of 31 samples for RNA-seq. The green and red dots represent individuals selected for RNA-seq. Twelve samples from each line by diet combination were selected for RT-qPCR validation of DEGs such that the corresponding RFI phenotypes were representative. The red and blue dots represent the samples selected for RT-qPCR assays. The 24 novel blood samples were selected such that the RFI values of the corresponding animals were roughly evenly distributed across the ranges of RFI not covered by the RFI phenotypes of the 24 animals originally selected for RNA-seq. The distribution of RFI values of barrows from each line by diet combination is also shown for reference

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