Fig. 1

Experimental design. Experimental design of the RNA-seq analysis is illustrated. Bipotential, sex fate commitment and sex differentiation period are indicated, with temperature sensitive period (TSP; indicated in light brown). The dotted line represents the end of the TSP. Eggs were first incubated under female producing temperature (FPT; indicated in red) until just prior to the onset of sexual differentiation (stage 19; Day 0), which were then either shifted to male producing temperature (MPT; indicated in blue) or kept at FPT. Gonadal regions were sampled from individuals at several subsequent time points (Day 3, 6, 12) with corresponding approximate developmental stage (Ferguson) displayed in the bottom table. Day 0–12 represents the timing of sexual differentiation, and three individuals per temperature condition per time points are used