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Fig. 1 | BMC Genomics

Fig. 1

From: A multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes

Fig. 1

Schematic representation of the methodology used to screen for (hemi) cellulases in fosmid-based metagenomic libraries. 1) Biased communities (e.g. soil-derived microbial consortia cultivated on wheat straw) are at the basis of enhanced hit rates. 2) Screening of fosmid pools (700 fosmids per pool) allow high-throughput analyses. 3) Screening on substrate mixtures accelerate the analyses. 4) Re-screening of positive clones in single, double and further mixed combinations of substrates enable the detection of specific activities

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BMC Genomics

ISSN: 1471-2164

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