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Table 1 Chromogenic substrates used in this study

From: A multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes

Activity ona

Type of enzymes detected

Substrate (indolyl-monosaccharide)

Abbreviation

Concentration into the plate

Supplier

Hemicellulose

alpha-fucosidases

5-bromo-4-chloro-3-indolyl-α-L-fucopyranoside

X-Fuc

Each 40 μg/ml

Biosynth AG, Switzerland

beta-galactosidases

5-bromo-4-chloro-indolyl-β-D-galactopyranoside

X-Gal

beta-xylosidases

5-bromo-4-chloro-3-indolyl-β-D-xylopyranoside

X-Xyl

alpha-mannosidases

5-bromo-4-chloro-3-indolyl α-D-mannopyranoside

X-Man

Cellulose and Starch

beta-glucanases

5-bromo-4-chloro-3-indolyl-β-D-cellobioside

X-Cel

alpha-glucosidases

5-bromo-4-chloro-3-indolyl-α-D-glucopyranoside

X-Glu

  1. aActivity was predicted based on the linked monosaccharide