A multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes

Table 2 Selected fosmids for insert sequencing and annotation of genes

				Number of genes based on RAST platform and CAZy database annotation
Fosmid ID	Positive on	# Contigs (total length)	% GC	GHs	CBMs	AAs	GTs	CEs	ABC	H/U	Others	Total
NT2-2	X-Gal	2 (31.21 Kb)	60.1	2	2	1	2	0	3	2	14	26
T5-5	X-Xyl	5 (31.63 Kb)	51.9	4	2	0	0	0	3	8	13	30
NT18-17	X-Glu	2 (33.7 Kb)	63.5	4	0	1	1	1	10	4	14	35
T4-1	Mixed^a	2 (34.84 Kb)	57.2	2	3	1	0	0	3	5	17	31
10BT	Mixed^a	2 (31.87 Kb)	54.6	2	0	2	1	0	1	12	25	43
NT18-21	X-Glu	1 (29.89 Kb)	55.0	0	1	0	0	0	3	2	21	27
T17-2	X-Gal	1 (23.57 Kb)	54.8	4	1	0	2	0	0	3	9	19

^apositive fosmids on the mixture of six chromogenic substrates, as in Table 1
GHs, glycosyl hydrolases, CBMs, carbohydrate binding modules, AAs, auxiliary activities, GTs, Glycosyl transferases,CEs, carbohydrate esterases ABC, ABC transporters, H/U, Hypothetical and unknown genes

ISSN: 1471-2164