Table 3 Carbohydrate-active genes detected in each fosmid insert
Fosmid ID | ID gene (Fig. 2) | CAZy Family a | Size in amino acids (kDa b) | Annotation based on RAST platform (EC number) | Probable protein from (identity / coverage) |
|---|---|---|---|---|---|
NT2-2 | 1 | CBM26 | 236 | GCN5-related N-acetyltransferase | Klebsiella oxytoca (73 % / 83 %) |
2 | GT2 | 521 | Uncharacterized protein YlaB | Enterobacter mori (58 % / 74 %) | |
3c | GH2 | 1028 (116) | Beta-galactosidase (EC 3.2.1.23) | Enterobacter hormaechei (74 % / 81 %) | |
4 | GH53 | 119 | Transcriptional repressor of the lac operon | Enterobacter hormaechei (59 % / 75 %) | |
5 | CBM48 | 301 | Carboxyl-terminal protease (EC 3.4.21.102) | Pseudomonas putida (99 % /100 %) | |
6 | AA3 | 565 | Choline dehydrogenase (EC 1.1.99.1) | Pseudomonas putida (99 % / 99 %) | |
7 | GT90 | 144 | Thioredoxin | Pseudomonas putida (95 % / 99 %) | |
T5-5 | 8 | GH32 | 310 | 6-Phosphofructokinase class II (EC 2.7.1.11) | Klebsiella oxytoca (92 % / 97 %) |
9 | GH13 | 241 | Ferric siderophore transport system, periplasmic binding protein TonB | Klebsiella pneumoniae (75 % / 84 %) | |
10 | CBM20 | 792 | Phosphoenolpyruvate synthase (EC 2.7.9.2) | Klebsiella oxytoca (98 % / 99 %) | |
11 | CBM50 | 154 | Probable lipoprotein NlpC precursor | Klebsiella oxytoca (98 % /99 %) | |
12c | GH3 | 789 (86) | Beta-xylosidase (EC 3.2.1.37) | Enterobacter mori (84 % /91 %) | |
13 | GH17 | 465 | Xyloside transporter XynT | Enterobacter cloacae (82 % / 90 %) | |
NT18-17 | 14c | GH27 | 218 (22) | Aquaporin Z | Hyphomonas neptunium (65 % / 80 %) |
15 | AA6 | 195 | NAD(P)H oxidoreductase | Rhizobium etli (48 % / 64 %) | |
16 | GH20 | 642 | Beta-hexosaminidase (EC 3.2.1.52) | Rhizobium leguminosarum 3841 (50 % / 64 %) | |
17c | GH58 | 356 (38) | Hypothetical protein | Hyphomicrobium denitrificans (56 % / 71 %) | |
18 | GH109 | 231 | Dehydrogenase | Rhizobium leguminosarum (58 % / 76 %) | |
19 | GT2 | 442 | Omega-amino acid-pyruvate aminotransferase (EC 2.6.1.18) | Mesorhizobium opportunistum WSM207 (82 % / 90 %) | |
20 | CE9 | 483 | Dihydropyrimidinase (EC 3.5.2.2) | Rhizobium leguminosarum (80 % / 90 %) | |
T4-1 | 21 | CBM50 | 154 | Probable lipoprotein NlpC precursor | Klebsiella oxytoca (92 % / 99 %) |
22 | CBM20 | 792 | Phosphoenolpyruvate synthase (EC 2.7.9.2) | Klebsiella oxytoca (98 % / 99 %) | |
23c | GH17 | 465 (52) | Xyloside transporter XynT | Enterobacter cloacae (82 % / 90 %) | |
24c | GH3 | 789 (86) | Beta-xylosidase (EC 3.2.1.37) | Enterobacter mori (83 % / 90 %) | |
25 | AA4/AA7 | 1000 | Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD | Klebsiella oxytoca (95 % / 98 %) | |
26 | CBM50 | 332 | L,D-transpeptidase YnhG | Klebsiella oxytoca (85 % / 93 %) | |
10BT | 27 | GT4 | 239 | Thioesterase involved in non-ribosomal peptide biosynthesis | Pseudomonas putida (90 % / 93 %) |
28 | AA3 | 413 | Sarcosine oxidase beta subunit (EC 1.5.3.1) | Pseudomonas putida (98 % / 99 %) | |
29c | GH39 | 192 (21) | Transcriptional regulator, AraC family | Klebsiella oxytoca (76 % / 87 %) | |
30 | AA5 | 127 | Integral membrane protein YfiB | Enterobacter cancerogenus (90 % / 94 %) | |
31c | GH53 | 406 (46) | Inner membrane protein YfiN | Enterobacter cloacae (79 % / 88 %) | |
NT18-21 | 32 | CBM50 | 396 | Shikimate 5-dehydrogenase I gamma (EC 1.1.1.25) | Klebsiella oxytoca (83 % / 90 %) |
T17-2 | 33 | GT8 | 207 | Galactoside O-acetyltransferase (EC 2.3.1.18) | Escherichia coli (81 % / 93 %) |
34c | GH2 | 1027 (116) | Beta-galactosidase (EC 3.2.1.23) | Citrobacter freundii (87 % / 92 %) | |
35 | GH53 | 360 | Transcriptional repressor of the lac operon | Citrobacter koseri (89 % / 94 %) | |
36 | CBM51 | 581 | Choline-sulfatase (EC 3.1.6.6) | Klebsiella oxytoca (94 % / 97 %) | |
37 | GH32 | 471 | PTS system, sucrose-specific IIB component | Klebsiella oxytoca (94 % / 97 %) | |
38 | GH65/GT5 | 266 | Cof, detected in genetic screen for thiamin metabolic genes | Klebsiella oxytoca (97 % / 98 %) | |
39 | GT4 | 330 | Lysophospholipase L2 (EC 3.1.1.5) | Klebsiella oxytoca (94 % / 97 %) |