Fig. 3

Regulation of histone acetylation and Brd4. a Analysis of the acetylated state of the peptide of histone H4 aa 4-17. The singly acetylated form increases during differentiation, while the hyperacetylated states decrease. Above the bars in red the ANOVA p-value (one tail test) among the different conditions is displayed. b Western blotting of Brd4, Oct4 βIII-tubulin and Lamin, this last one used as control. Brd4 decreases during differentiation, and so Oct4. On the bottom, antibody recognizing acetylated histone H4. c qRT-PCR of Oct4 (Pou5f1, in black), Brd2 (in red) and Brd4 (in green) across the five time points of the differentiation process. Actin was used as the reference gene