Table 2 Primers used for qPCR
Gene name | Primer name | Primer sequence (5’ → 3’) | Product size | Acc Number |
|---|---|---|---|---|
Elongation factor-1α | EF1α-F | CAAGGATATCCGTCGTCGTGGCA | 327 | AF498320 |
EF1α-R | ACAGCGAAACGACCAAGAG | |||
DNA directed RNA | DRPII-F | TCACCCATGAAGTTGATGAGCTGA | 176 | BT073753 |
polymerase III subunit I | DRPII-R | CCGTGCAGACATAGTACAGCCTCA | ||
Hypoxanthine | HPRT1-F | GCCTCAAGAGCTACTGCAATG | 256 | ACH70616 |
Phosphoribosyltransferase 1 | HPRT1-F | GTCTGGAACCTCAAATCCTATG | ||
Thioredoxin reductase 3a | TrxR3a-F | AGTCAACCCCAAGAACGGTAAGG | 297 | HF969246 |
TrxR3a-R | CAGAAGAGACTGTGGTACACCTCCAA | |||
Thioredoxin reductase 3b | TrxR3b-F | CAAAGTCAACCCCAAGAATGGTAAGA | 300 | HF969247 |
TrxR3b-R | CAGAAGAGACTGTGGTACACCTCCAG | |||
Selenoprotein Pa | SelPa-F | GCTTGGTGCAGGCATCCTTATTG | 276 | HF969249 |
SelPa-R | CATATCTCCCTGCCCTACTCCATCC | |||
Selenoprotein Pb | SelPb-F | GACGACTTCCTGGTATATGACAGATGTG | 275 | HF969250 |
SelPb-F1 | GATACCGTCAGCAACCCAGTTCC | |||
Interferon 1a | IFN-1aF | CTGTTTGATGGGAATATGAAATCTGC | 193 | AJ580911 |
IFN-1aR | CCTGTGCACTGTAGTTCATTTTTCTCAG | |||
Interferon 1b | IFN-1bF | GATGGGAATAGGAATAGGAATAGGAAGTC | 200 | AJ582754 |
IFN-1bR | GCCTCTGCACTGTAGTTCATTTTTCTC | |||
Interferon γ1 | IFN-γ1F | CAAACTGAAAGTCCACTATAAGATCTCCA | 210 | AJ616215 |
IFN-γ1R | TCCTGAATTTTCCCCTTGACATATTT | |||
Interferon γ2 | IFN-γ2F | CAAACTGAAAGTCCACTATAAGATCTCCA | 188 | FM864345 |
IFN-γ2R | GGTCCAGCCTCTCCCTCAC | |||
Interferon γ induced protein | CXCL11-F | CATCAGCTTCCTGGCCTGTC | 187 | AJ417078 |
CXCL11-R | CCGTTCTTCAGAGTGACAATGATCTC | |||
Viperin | VIG-F | AGAACTCAACCCTGTACGCTGGA | 227 | AF076620 |
VIG-R | GGCAATCCAGGAAACGCATATATTC | |||
Laboratory of genetics | LGP2-F | CAGGGACTTCCGAATGAAGATCAC | 230 | FN396358 |
and physiology 2 | LGP2-R | CGCCGGTCTTATAGTACCTCTCAAAGTC | ||
Melanoma differentiation- | MDA5-F | CCTTTTCACGCTCTTTAAAGATAGCAAAG | 229 | FN396357 |
associated gene 5 | MDA5-R | GCAAGCTTTTACTCCGACCTCCTC | ||
Toll-like receptor 3 | TLR3-F | GAACGTTCTGATCAACCGTACGCT | 297 | AY883999 |
TLR3-R | TGGGCCATGAACTGTCGACA | |||
Toll-like receptor 9 | TLR9-F | GGCTGCTGGATGAAAAGGTGGA | 212 | EU627195 |
TLR9-R | CTCGTTGACGTTGCTGTCGTAGGA | |||
MX protein 2 | MX2-F | CCTTCTGAAAACAGCAAAGACTAAGA | 184 | OM47945 |
MX2-R | AACTAACTCTCCCTCCTCCAACTC | |||
Serum amyloid A | SSA-F | GGTGAAGCTGCTCAAGGTGCTAAAG | 162 | AM422447 |
SSA-R | GCCATTACTGATGACTGTTGCTGC | |||
Suppressor of cytokine | SOCS3-F | CACAGAGAAACCGTTAAAAGGACTATCC | 228 | AM748723 |
signalling 3 | SOCS3-R | AAGGGGCTGCTGCTCATGAC | ||
Cathelicidin 1 | CATH1-F | ACCAGCTCCAAGTCAAGACTTTGAA | 275 | AY594646 |
CATH1-R | TGTCCGAATCTTCTG CTGCAA | |||
Hepcidin | HAMP-F | GCTGTTCCTTTCTCCGAGGTGC | 165 | CA369786 |
HAMP-R | GTGACAGCAGTTGCAGCACCA | |||
Insulin-like growth factor | IGFBP-1b1F | ATCCCAGACCCCTCCACTCC | 258 | JX565545 |
binding protein | IGFBP-1b1R | GCTGAGAGCTGGTTATCTTGTCC |