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Fig. 1 | BMC Genomics

Fig. 1

From: Uncovering the pathways underlying whole body regeneration in a chordate model, Botrylloides leachi using de novo transcriptome analysis

Fig. 1

Staging scheme used for WBR in B. leachi. a Stage A: B. leachi colony prior to dissection. Black dashed line indicates the dissection sites. Stage 0: Marginal ampullae at 0 h, directly after dissection from the zooids. Black dashed line indicates dissection site. Stage 1: New vascular connections formed between ampullae, creating the beginning of a new circulatory system. Stage 2: Marginal ampullae starting to condense together, creating a compact network of blood vessels within the tunic matrix. Stage 3: Further condensing of the blood vessels. Stage 4: Formation of small transparent vesicle (regeneration niche) in the middle of the condensed blood vessels. The regeneration niche continues to expand in size, gaining pigmentation and ultimately forming the new adult. Stage 5: A fully developed zooid capable of filter feeding forms ~ 8 days. b Higher magnification image of the terminal ampullae at Stage 0. Red line surrounding individual ampullae and double arrow indicating space between two ampullae. This distance inversely correlates with the time it takes the vascular tissue to reach Stage 3. c Same as b, with arrows pointing at blood vessels connecting individual ampullae to one another. d–f Higher magnification images of Stage 4. Yellow line surrounding the regeneration niche that grows to form the new adult. g Adult zooid capable of filter feeding. Arrows indicating the two siphons present (atrial and peribranchial siphons). Scale bar represents 0.5 mm

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